Cette publication parue sur la revue Journal
of Invertebrate pathology est issue d'une collaboration avec une équipe de Taïwan spécialisés sur Nosema ceranae, avec laquelle j'ai travaillé en 2005 et 2006, dès les premières annonces sur la
possibilité d'existence de Nosema ceranae en Europe.
La publication complète est disponible chez Elsevier : www.elevier.com
D'autres travaux sont en cours sur ce même sujet.
The comparison of rDNA spacer regions of
Nosema ceranae isolates from different hosts and locations q
a, Michel Bocquet b, Ker-Chang Lee a, I-Hsin Sung c, Jing-Hao Jiang a, Yue-Wen Chen d, Chung-Hsiung Wang a,*
Department of Entomology, National Taiwan University, No. 1, Sec. 4, Roosevelt Rd., 106 Taipei, Taiwan
Individual Researcher, BP 22, 74371 PRINGY Cedex, France
Tainan District Agricultural Research and Extension Station, Council of Agriculture, Executive Yuan, 712 Tainan, Taiwan
Department of Animal Science, National I-Lan University, I-Lan 260, Taiwan
Received 12 October 2006; accepted 9 July 2007, Available online 18 July 2007
is a common microsporidian pathogen, one of two Nosema species that cause ‘‘nosema disease’’ in honeybees, Apis cerana and
Apis mellifera. Samples of N. ceranae rDNA from isolates
collected in different locations were sequenced and one 5S rRNA was found to be upstream of SSUrRNA. The rDNA arrangement, 50-5S rRNA–IGS–SSUrRNA–ITS–LSUrRNA-30, was found in all isolates.
In order to better understand the distribution relationship between N. ceranae isolates
from A. cerana and A. mellifera, their rRNA
spacer regions were also sequenced for analysis. Results showed that there are no significant differences between the IGS sequences of the isolates and no difference in the ITS sequence with the
exception of one transition found in an isolate from Martinique. These isolates showed consistency in the IGS phylogenic analysis suggesting that no transmission barrier exists
between A. mellifera and A. cerana and there is no
difference between isolates from geography separated areas.
2007 Elsevier Inc. All rights reserved.
Keywords: Nosema ceranae
; Nosema disease; rDNA; IGS; ITS; France; Martinique; Spain; Taiwan