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  • : Le blog de Michel BOCQUET
  • Le blog de Michel BOCQUET
  • : Michel BOCQUET, Ingenieur agronome, consultant en apiculture ; conseil et expertise dans les domaines liés à l'abeille, les produits de la ruche, la pollinisation, l'utilisation des abeilles comme bioindicateurs de l'environnement. Michel BOCQUET, Agronomist, apiculturist : specialist in honeybees, bee products, pollination, environment monitoring with bees as bio indicator.
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Cette publication parue sur la revue Journal of Invertebrate pathology est issue d'une collaboration avec une équipe de Taïwan spécialisés sur Nosema ceranae, avec laquelle j'ai travaillé en 2005 et 2006, dès les premières annonces sur la possibilité d'existence de Nosema ceranae en Europe.

La publication complète est disponible chez Elsevier : www.elevier.com

D'autres travaux sont en cours sur ce même sujet.


The comparison of rDNA spacer regions of

 

Nosema ceranae isolates from different hosts and locations q

Wei-Fone Huang

 

a, Michel Bocquet b, Ker-Chang Lee a, I-Hsin Sung c, Jing-Hao Jiang a, Yue-Wen Chen d, Chung-Hsiung Wang a,*

a

 

Department of Entomology, National Taiwan University, No. 1, Sec. 4, Roosevelt Rd., 106 Taipei, Taiwan

b

 

Individual Researcher, BP 22, 74371 PRINGY Cedex, France

c

 

Tainan District Agricultural Research and Extension Station, Council of Agriculture, Executive Yuan, 712 Tainan, Taiwan

d

 

Department of Animal Science, National I-Lan University, I-Lan 260, Taiwan

Received 12 October 2006; accepted 9 July 2007, Available online 18 July 2007

Abstract

Nosema ceranae

 

is a common microsporidian pathogen, one of two Nosema species that cause ‘‘nosema disease’’ in honeybees, Apis cerana and Apis mellifera. Samples of N. ceranae rDNA from isolates collected in different locations were sequenced and one 5S rRNA was found to be upstream of SSUrRNA. The rDNA arrangement, 50-5S rRNA–IGS–SSUrRNA–ITS–LSUrRNA-30, was found in all isolates. In order to better understand the distribution relationship between N. ceranae isolates from A. cerana and A. mellifera, their rRNA spacer regions were also sequenced for analysis. Results showed that there are no significant differences between the IGS sequences of the isolates and no difference in the ITS sequence with the exception of one transition found in an isolate from Martinique. These isolates showed consistency in the IGS phylogenic analysis suggesting that no transmission barrier exists between A. mellifera and A. cerana and there is no difference between isolates from geography separated areas.

2007 Elsevier Inc. All rights reserved.

Keywords: Nosema ceranae

 

; Nosema disease; rDNA; IGS; ITS; France; Martinique; Spain; Taiwan

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